Analysis of HIV-2 indeterminate Western blot patterns showed the frequency of appearance of antibodies to the various viral gene products in 396 HIV-2 indeterminate sera. Antibodies for the GAG gene products, i.e., anti-55, anti-p24, and anti-p18 had high frequencies of occurrence with anti-p24 occurring 90.5% of the time. Analysis of HIV-1 indeterminate Western blot patterns showed the frequency of protein bands for 231 HIV-1 indeterminate sera. HIV-2 GAG gene product p26 was shown to be a non-specific indicator of infection. Antibodies to group specific antigen (GAG) gene products were most frequently detected both HIV-1 and HIV-2 indeterminate sera.
Sera and plasma after screening by enzyme-linked immunosorbent assay (ELISA) were used. In both cases, proteins of the specific virus type were used to detect anti-HIV proteins in sera by the enzyme linked immunoelectrotransfer blot (Western blot) technique. New LavblotII kits were used for detecting HIV-2 antibodies. For HIV-1 antibodies detection, Novopath Immunoblot assay kits were used. Antibody to gp120, and envelope gene product of HIV-1 never occurred in indeterminate sera whereas antibodies to all the envelope gene products of HIV-2 were detected in indeterminate sera.Ī comparison of HIV-1 and HIV-2 indeterminate Western blot patterns of Ghanaian sera collected between 19 was made and interpreted according to new World Health Organization (WHO) criteria. A comparison of HIV-1 and HIV-2 indeterminate Western blot patterns of Ghanaian sera collected between 19 was made.
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